Phlebotomy Technician Practice Exam (NCPT/CPT)

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What is the Order of Draw for all additive tubes?

  1. SST, Light Blue, Lavender, Green, Grey

  2. Light Blue, SST, Green, Lavender, Grey

  3. Green, Lavender, Grey, Light Blue, SST

  4. Lavender, Grey, Light Blue, SST, Green

The correct answer is: Light Blue, SST, Green, Lavender, Grey

The correct order of draw for all additive tubes is Light Blue, SST, Green, Lavender, Grey. This is because each tube has a specific additive that must be drawn in the correct order to avoid cross contamination. The first tube, Light Blue, contains a sodium citrate additive which prevents blood from clotting. The following SST tube will contain a clot activator and gel for serum separation. If the SST tube is drawn before the Light Blue, the clot activator could potentially interfere with the sodium citrate and affect coagulation results. Green tubes contain the anticoagulant heparin and should be drawn before Lavender (EDTA) tubes to avoid any potential contamination. Lavender tubes are used for hematology testing and the last tube, Grey, contains potassium oxalate which is used for glucose testing. Drawing the Grey tube before the Lavender tube could potentially affect blood

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